Alcohol-based solutions for bovine testicular tissue fixation

Cryopreservation of Testicular Tissue

Immediate use of freshly collected testis tissue in diagnosis or in reproductive technologies is not always possible or desirable. Therefore, the ability to properly preserve the tissue for varying intervals is an essential step for maximizing the use of the source tissue. Preservation of gametes and gonads is a topic of interest in reproductive biomedicine. Other chapters in this book have ele...

Ethanolic fractionation of bovine testicular hyaluronidase.

Studies on the fractionation of blood serum by Cohn et al. (1) have demonstrated that under precisely controlled conditions reproducible fractionation of a complex mixture of proteins can be accomplished by the use of ethanol. This paper reports the application of this method to the separation of hyaluronidase from testicular extract. A variety of experimental conditions involving variation of ...

Mechanism of Action of Bovine Testicular Hyaluronidase

The reactions of purified, homogeneous bovine testicular hyaluronidase have been studied with radioactively labeled oligomers of hyalobiuronic acid, (GlcUA-GlcNAc),, as substrates and acceptors. Transglycosylation occurs by transfer of a glycosyl residue with retention of configuration from a leaving group to an acceptor. On the basis of detailed examination of cleavage and transglycosylation p...

Long-Term Effect of Ciprofloxacin on Testicular Tissue: Evidence for Biochemical and Histochemical Changes

Comparison of Tissue Stiffness Using Shear Wave Elastography in Men with Normal Testicular Tissue, Testicular Microlithiasis and Testicular Cancer

Objectives To compare elastography measurements in men with normal testicular tissue, testicular microlithiasis and testicular cancer. Methods A total of 248 consecutive patients were included. All men provided written informed consent. Testicular stiffness was assessed using shear wave elastography (SWE). Three SWE velocity measurements were assessed in each testicle. The patients were divid...